TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: Mab11]

CAT#: AM26711PU-N

TNF alpha (TNF) mouse monoclonal antibody, clone Mab11, Aff - Purified

Specifications

Product Data

• Clone Name: Mab11
• Applications: FC, FN, IF, IHC
• Recommended Dilution: Flow cytometry: Intracellular staining
  Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections.
  Immunocytochemistry.
  Functional application: Neutralization.
• Reactivities: Human, Porcine, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human TNF-alpha
• Specificity: The mouse monoclonal antibody MAb11 recognizes 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
• Formulation: Phosphate buffered saline (PBS)
  State: Aff - Purified
  State: Liquid Ig fraction
  Preservative: 15 mM sodium azide, approx. pH 7.4
• Concentration: lot specific
• Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: tumor necrosis factor
• Database Link:
  Entrez Gene 7124 Human
• Background: TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin