Claudin 6 (CLDN6) Rat Monoclonal Antibody [Clone ID: YD-9H8]

CAT#: AM26752PU-N

Claudin 6 (CLDN6) rat monoclonal antibody, clone YD-9H8, Purified

Product Images

SDS-PAGE analysis of purified YD-4E9 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified YD-4E9 antibody. Proteins were separated by SDS-PAGE and stained with RAPID Stain TM Reagent.

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  BOSC23 cells were transiently transfected with an expression vector encoding either Claudin 9 (red curve), Claudin 6 (green curve) or an irrelevant protein (control transfectant). Binding of YD-9H8 was detected with a PE conjugated secondary antibody. A positive signal was obtained with Claudin 9 and Claudin 6 transfected cells.

Specifications

Product Data

• Clone Name: YD-9H8
• Applications: ELISA, FC, WB
• Recommended Dilution: Flow cytometry: 1.2 µg/10⁶ cells.
  ELISA: 1/200-1/400.
  CELISA: 1/200-1/400.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: cDNA encoding Human Claudin 9.
  Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.
• Specificity: This antibody detects Human Claudin-6 and Claudin-9.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: None
• Concentration: 2.0 mg/ml
• Purification: Protein G Chromatography
• Gene Name: claudin 6
• Database Link:
  Entrez Gene 9074 Human
• Background: Claudin 6 and Claudin 9 belong to the claudin family which constitutes a large group of four - transmembrane domain proteins (1) . Claudins are integral tight junction proteins that are responsible for maintaining the integrity of epithelial cell architecture, the control of parace llular transport and cell polarity. The expression pattern of claudins is tissue specific, most tissues express multiple claudins, which can interact in both homotypic and heterotypic fashion to form the tight junction strands (2) . Several claudin proteins have been shown to be abnormally expressed in cancers. The differential expression of these proteins between tumour and normal cells, in addition to their membrane localisation, makes them prime candidates for cancer therapy (2). Members of the claudin fa mily, including Claudin 6 and 9 are expressed in the liver and play a critical role in Hepatitis C Infection. They function as coreceptors in HCV entry and assume a role in HCV dissemination, replication and pathogenesis (3).
• Synonyms: CLDN6, PRO1488, Skullin, Skullin 2, UNQ757, CLDN9