IL2 Receptor alpha (IL2RA) Mouse Monoclonal Antibody [Clone ID: 143-13]

CAT#: AM26760BT-N

IL2 Receptor alpha (IL2RA) mouse monoclonal antibody, clone 143-13, Biotin


USD 455.00

2 Weeks*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name 143-13
Applications FC, IHC
Recommended Dilution Immunohistochemistry on frozen sections: 10mg/ml (1:40).
Suggested positive control: Human tonsil.
Has been described to work in FACS.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Stimulated human leukocytes
Specificity This antibody detects CD25, a 55kD glycoprotein. It recognizes epitope region C of the IL-2 receptor complex.
Formulation Phosphate buffered saline pH 7.2
Label: Biotin
State: Lyophilized Ig fraction
Stabilizer: 5 mg/ml bovine serum albumin (BSA)
Preservative: 0.05% (v/v) Kathon CG
Reconstitution Method Reconstitute by adding 0.5ml distilled water.
Concentration 0.4 mg/ml (reconstituted)
Purification Affinity purified
Conjugation Biotin
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background IL-2R (T-cell growth factor receptor) consists of a chain of 55kDa (also known as Tac antigen or p55 or α chain) and a p75 or β chain. IL-2Rα is over-expressed on the surface of activated Tcells, HTLV-1 or -2 positive T-cell lines, and moderately expressed on activated B-cells, NK-cells, and macrophages. CD25 molecule alone appears to function as a low affinity receptor and associates wtih CD122 (IL-2R β chain, p75) and CD132 (common γ chain) to form the high affinity IL-2 receptor complex.
Synonyms Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen
Note Antigen distribution:
Isolated cells: The antibody stains less than 5% of resting human peripheral blood mononuclear cells in flow cytometry.
Tissue sections: Scattered large and small lymphocytes in germinal centers, in follicle mantle, and T-zone.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.