IL2 Receptor alpha (IL2RA) Mouse Monoclonal Antibody [Clone ID: 143-13]

CAT#: AM26760BT-N

IL2 Receptor alpha (IL2RA) mouse monoclonal antibody, clone 143-13, Biotin

Product Images

: Human tonsil, frozen sections.

Specifications

Product Data

• Clone Name: 143-13
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on frozen sections: 10mg/ml (1:40).
  Suggested positive control: Human tonsil.
  Has been described to work in FACS.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Stimulated human leukocytes
• Specificity: This antibody detects CD25, a 55kD glycoprotein. It recognizes epitope region C of the IL-2 receptor complex.
• Formulation: Phosphate buffered saline pH 7.2
  Label: Biotin
  State: Lyophilized Ig fraction
  Stabilizer: 5 mg/ml bovine serum albumin (BSA)
  Preservative: 0.05% (v/v) Kathon CG
• Reconstitution Method: Reconstitute by adding 0.5ml distilled water.
• Concentration: 0.4 mg/ml (reconstituted)
• Purification: Affinity purified
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3559 Human
• Background: IL-2R (T-cell growth factor receptor) consists of a chain of 55kDa (also known as Tac antigen or p55 or α chain) and a p75 or β chain. IL-2Rα is over-expressed on the surface of activated Tcells, HTLV-1 or -2 positive T-cell lines, and moderately expressed on activated B-cells, NK-cells, and macrophages. CD25 molecule alone appears to function as a low affinity receptor and associates wtih CD122 (IL-2R β chain, p75) and CD132 (common γ chain) to form the high affinity IL-2 receptor complex.
• Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen
• Note: Antigen distribution:
  Isolated cells: The antibody stains less than 5% of resting human peripheral blood mononuclear cells in flow cytometry.
  Tissue sections: Scattered large and small lymphocytes in germinal centers, in follicle mantle, and T-zone.