EPOR (Cytoplasm. Dom.) Rat Monoclonal Antibody [Clone ID: BCO-3H2]

CAT#: AM26787PU-N

EPOR (Cytoplasm. Dom.) rat monoclonal antibody, clone BCO-3H2, Purified

Product Images

Mass spectroscopic analysis of EpoR immuno - precipitated from UT7 cells by AM26787PU-N or other antibody that were further analysed on denaturing SDS - PAGE gels (identified in Western blot with AM26787PU-N). Segments of the gel were cut out for analysis by mass spectroscopy. For detailed information see Reference 5.

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  CGE analysis of purified AM26787PU-N monoclonal antibody.Lane 1: molecular weight marker, Lane 2: 2 ug of purified AM26787PU-N antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4, 5 kDa).

Specifications

Product Data

• Clone Name: BCO-3H2
• Applications: ELISA, FC, WB
• Recommended Dilution: Flow Cytometry only on permeabilised cells: 1.2 μg/10⁶ cells.
  ELISA: 1/200-1/400.
  Cell based ELISA only on permeabilised cells: 1/200-1/400.
  Western blot: See ref.5.
  Immunohistochemistry: See ref.5.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Peptide immunisation from the cytoplasmic domain of human Epo-R
• Specificity: This antibody detects Human Epo-R.
  Other species not tested.
• Formulation: Phosphate buffered saline, pH 7.2
  State: Purified
  State: Liquid Ig fraction
• Purification: Protein G affinity chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2057 Human
• Background: Erythropoietin receptor (Epo-R) belongs to the cytokine receptor family and is a 507 amino acid type I transmembrane protein. EpoR pre-exists as dimers which changes the homodimerized state after binding of its 34 kDa ligand erythropoietin (Epo) (1,2). Erythropoietin is the primary reg ulator of erythropoiesis, and promotes the survival, proliferation, and differentiation of erythroid progenitor cells. Both, Epo and Epo-R are essential for the production of red blood cells due to Epo exerts its function through the E po receptor (3). The Epo-R is also expressed in many organs outside the bone marrow, suggesting that Epo is a pleiotropic anti-apoptotic factor. Signaling pathways have been shown to influence numerous cellular functions in normal and tumor cells, including proliferation, apoptosis, and drug resistance (4). Development and specificity testing of the antibody are described in detail elsewhere (5).
• Synonyms: EPOR, EPO-R