Progesterone Receptor (PGR) Mouse Monoclonal Antibody

CAT#: AM31005PU-N

Progesterone Receptor (PGR) mouse monoclonal antibody, Purified

Product Images

Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: Immunofluorescence.
  Immunohistochemistry on Paraffin Sections: 1/50.
  Immunoprecipitation (NATIVE ONLY): 2 µg/mg protein lysate Using Protein G.
  Western Blot: 1 - 2 µg/ml for 2 hrs at RT.
  Positive control: T47D cells or breast carcinoma.
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human endometrial carcinoma (EnCa 101) grown in athymic mice.
• Specificity: Equally recognizes two proteins: 116kD (triplet) and 81kD (singlet) which are identified as the hormone-binding high (B) and low (A) MW forms of Human progesterone receptor. Does not cross-react with Guinea Pig.
• Formulation: PBS, pH 7.4 containing 0.2% BSA as stabilizer and 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 0.2 mg/ml
• Purification: Protein G Chromatography
• Gene Name: Homo sapiens progesterone receptor (PGR), transcript variant 2
• Database Link:
  Entrez Gene 18667 MouseEntrez Gene 25154 RatEntrez Gene 5241 Human
• Background: The progesterone receptor, a NR3 Steroid Receptor, is induced in granulosa cells of preovulatory follicles in response to surges in luteinizing hormone. It is essential for ovulation and egg implantation. A decrease in expression of endometrial progesterone receptor results in infertility in women. This receptor mediates the progesterone-induced transcription of proteases including ADAMTS1 and cathepsin L. In breast cancer, progesterone receptors are associated with hormone dependence and prolonged survival. At least two alternatively spliced isoforms have been identified in human: hPR-A (94 kD) is 164 aa shorter than hPR-B (114 kD). In most cell contexts, hPR-B functions as a transcriptional activator of progesterone-responsive genes, whereas hPR-A functions as a transcriptional inhibitor of all steroid hormone receptors. hPR-A and hPR-B have been shown to regulate different sets of genes in human breast cancer cells. Some of these PR-regulated genes are implicated in breast cancer.
• Synonyms: PR, PGR, NR3C3

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Oocyte meiosis, Progesterone-mediated oocyte maturation