MEK3 (MAP2K3) (1-100) Mouse Monoclonal Antibody [Clone ID: 1D10]

CAT#: AM31038PU-N

MEK3 (MAP2K3) (1-100) mouse monoclonal antibody, clone 1D10, Purified

Product Images

Detection limit for recombinant GST tagged MAP2K3 is approximately 0.03ng/ml as a capture antibody.

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  Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 1D10
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: MAP2K3 / MEK3 / MKK3 antibody was raised against mAP2K3 (AAH32478, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag.
  MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to MAP2K3 / MEK3 at aa 1-100.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Protein A chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant A
• Database Link:
  Entrez Gene 5606 Human
• Background: MEK3, a MAP2K type protein kinase, is a member of the p38 MAP kinase signal transduction pathway which mediates stress induced cell cycle arrest, transcription activation and apoptosis. MEK3 can be activated by insulin and is required for glucose transporter expression. The protein is phosphorylated by ASK1 or TAO1, and in turn, it phosphorylates the MAPKs p38-alpha and -beta. At least three protein isoforms, resulting from alternatively spliced transcript variants of the MEK3 gene, have been identified - 318, 347 and 352 amino acids. Oncogenic transformation of primary cells has been linked to accumulation of activated MEK3 which results from expression of the RAS oncogene. Yersina pseudotuberculosis has been shown to block phosphorylation and subsequent activation of MEK3.
• Synonyms: MAPKK 3, MEK3, MKK3, MAP kinase kinase 3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway