ENO3 Mouse Monoclonal Antibody [Clone ID: 5D1]

CAT#: AM31050PU-N

ENO3 mouse monoclonal antibody, clone 5D1, Purified

Product Images

ENO3 monoclonal antibody (M01), clone 5D1 Western Blot analysis of ENO3 expression in HeLa.

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  Western Blot analysis of ENO3 expression in transfected 293T cell line by ENO3 monoclonal antibody (M01), clone 5D1.

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  Western blot analysis of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody (M01), clone 5D1.

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  Detection limit for recombinant GST tagged ENO3 is approximately 0.03ng/ml as a capture antibody.

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  Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to ENO3 on HeLa cell. [antibody concentration 10 ug/ml]

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Specifications

Product Data

• Clone Name: 5D1
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: ENO3 antibody was raised against recombinant protein
• Specificity: This antibody reacts to ENO3.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Protein A chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens enolase 3 (ENO3), transcript variant 3
• Database Link:
  Entrez Gene 2027 Human
• Synonyms: Muscle-specific enolase, Skeletal muscle enolase, Enolase 3, ENO3, ENOB

Reference Data

• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation