IRE1 (ERN1) Mouse Monoclonal Antibody [Clone ID: 3D1]

CAT#: AM31059PU-N

IRE1 (ERN1) mouse monoclonal antibody, clone 3D1, Purified

Product Images

ERN1 monoclonal antibody clone 3D1 Western Blot analysis of ERN1 expression in HeLa.

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  Detection limit for recombinant GST tagged ERN1 is approximately 0.3ng/ml as a capture antibody.

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  Human Prostate, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 3D1
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: ERN1 antibody was raised against recombinant protein
• Specificity: This antibody reacts to ERN1 / IRE1.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Protein A chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens endoplasmic reticulum to nucleus signaling 1 (ERN1)
• Database Link:
  Entrez Gene 2081 Human
• Background: The endoribonuclease IRE1/ERN1 encodes the ER-to-nucleus signaling 1 protein, a human homolog of the yeast Ire1 gene product. In S. cerevisiae, as well as in human, this ER transmembrane IRE serine/threonine protein kinase monitors the status of unfolded proteins inside the ER lumen in response to stress. Activation of ERN1 leads to oligomerization, autophosphorylation, and proteolytic cleavage. The kinase and nuclease domains are then transported into the nucleus to function as a site-specific endoribonuclease. ERN1 activates JNK and other chaperone genes in response to stress.
• Synonyms: Inositol-requiring protein 1, IRE1p, Ire1-alpha, IRE1a

Reference Data

• Protein Families: Protein Kinase, Transmembrane
• Protein Pathways: Alzheimer's disease