LIM Kinase 1 (LIMK1) Mouse Monoclonal Antibody [Clone ID: 2E9]

CAT#: AM31074PU-N

LIM Kinase 1 (LIMK1) mouse monoclonal antibody, clone 2E9, Purified

Product Images

LIMK1 monoclonal antibody (M05), clone 2E9 Western Blot analysis of LIMK1 expression in HeLa.

• 

  Detection limit for recombinant GST tagged LIMK1 is approximately 0.3ng/ml as a capture antibody.

• 

  Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 2E9
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: LIMK1 antibody was raised against recombinant protein
• Specificity: This antibody reacts to LIMK1.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Protein A chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 2
• Database Link:
  Entrez Gene 3984 Human
• Background: LIMK1, a LIMK -type protein kinase, is a component of the cellular motility machinery regulating actin filament dynamics. The protein is phosphorylated by the coiled-coil-containing protein kinase ROCK, a downstream effector of the GTPase Rho. Activated LIMK1 phosphorylates cofilin and thereby inhibits its actin-depolymerizing activity. At least two protein isoforms of 305 and 647 amino acids have been reported. LIMK1 hemizygosity has been associated with the impaired visuospatial constructive cognition of Williams syndrome.
• Synonyms: LIMK, LIM domain kinase 1, LIMK-1, EC=2.7.11.1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton