KAP1 (TRIM28) Mouse Monoclonal Antibody [Clone ID: 4E6]

CAT#: AM31084PU-N

KAP1 (TRIM28) mouse monoclonal antibody, clone 4E6, Purified

Product Images

TRIM28 monoclonal antibody, clone 4E6. Western Blot analysis of TRIM28 expression in PC-12.

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  Western Blot analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody, clone 4E6.

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  Western blot analysis of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody clone 4E6. GAPDH ( 36.1 kDa ) used as control.

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  Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to TRIM28 on HeLa cell. [antibody concentration 10 ug/ml]

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Specifications

Product Data

• Clone Name: 4E6
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag.
• Specificity: This antibody recognizes TRIM28.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purfied Ig fraction
• Concentration: 0.5 mg/ml
• Purification: Protein A Chromatography
• Gene Name: Homo sapiens tripartite motif containing 28 (TRIM28)
• Database Link:
  Entrez Gene 21849 MouseEntrez Gene 116698 RatEntrez Gene 10155 Human
• Background: TIF1beta is a member of the Transcriptional Intermediary Factor 1 (TIF1) subfamily. TIF1beta contains a RING finger, B box, Coiled coil, PHD/TTC, and bromodomain. TIF1beta is a corepressor for Kruppel-associated box (KRAB)-domain-containing zinc finger proteins and plays a critical role in early embryogenesis. TIF1beta acts as a transcriptional mediator by binding liganded nuclear receptors, including retinoic acid (RAR), retinoid X (RXR) and estrogen (ER) receptors. TIF1beta associates with both heterochromatin and euchromatin, causing gene silencing by both HP1 binding and histone deacetylation.
• Synonyms: TIF1 beta, TIF1B, KAP1, KAP-1, KRIP1, KRIP-1, RNF96, RING finger protein 96

Reference Data

• Protein Families: Protein Kinase, Stem cell - Pluripotency, Transcription Factors