SMAD3 Mouse Monoclonal Antibody [Clone ID: 7F3]

CAT#: AM31100PU-N

SMAD3 mouse monoclonal antibody, clone 7F3, Purified

Product Images

SMAD3 monoclonal antibody clone 7F3. Western Blot analysis of SMAD3 expression in human colon.

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  SMAD3 monoclonal antibody clone 7F3. Western Blot analysis of SMAD3 expression in HeLa.

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  SMAD3 monoclonal antibody clone 7F3. Western Blot analysis of SMAD3 expression in Jurkat.

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  Detection limit for recombinant GST tagged SMAD3 is approximately 0.1ng/ml as a capture antibody.

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  Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

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Specifications

Product Data

• Clone Name: 7F3
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: SMAD3 antibody was raised against recombinant protein
• Specificity: This antibody reacts to SMAD3.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens SMAD family member 3 (SMAD3), transcript variant 2
• Database Link:
  Entrez Gene 25631 RatEntrez Gene 4088 Human
• Background: SMAD3, a transcriptional regulator, is activated by TGF-beta and Activin 1 receptor kinase. SMAD3 recognizes an 8 bp palindromic sequence GTCTAGAC. Upon phosphorylation at two extreme C-terminal serines, SMAD3 is translocated into the nucleus where is forms complexes other SMAD proteins, depending on the activation pathway, and regulates the transcription of target genes.
• Synonyms: SMAD family member 3, SMAD 3, SMAD-3, Mad3, MAD-3, MADH3, MAD homolog 3, JV15-2

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway