SMURF1 (165-269) Mouse Monoclonal Antibody [Clone ID: 1D7]

CAT#: AM31112PU-N

SMURF1 (165-269) mouse monoclonal antibody, clone 1D7, Purified

Product Images

Western Blot analysis of SMURF1 expression in Raw 264.7 cells with SMURF1 monoclonal antibody, clone 1D7.

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  Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody, clone 1D7.

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  Detection limit for recombinant GST tagged SMURF1 is approximately 0.1ng/ml as a capture antibody.

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  Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. [antibody concentration 10 ug/ml]

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  Immunoprecipitation of SMURF1 transfected lysate using anti-SMURF1 monoclonal antibody and Protein A Magnetic Bead.

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Specifications

Product Data

• Clone Name: 1D7
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Immunoprecipitation.
  Western Blot.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: SMURF1 (NP_065162, 165 a.a. ~ 269 a.a) partial recombinant protein with GST tag
• Specificity: This antibody reacts to SMURF1 at aa 165-269.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 0.26 mg/ml
• Purification: Affinity chromatography on Protein A
• Gene Name: Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1
• Database Link:
  Entrez Gene 75788 MouseEntrez Gene 57154 Human
• Background: E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA.
• Synonyms: SMURF 1, SMURF-1, KIAA1625

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis