PAX5 Mouse Monoclonal Antibody [Clone ID: 8F9]

CAT#: AM31120PU-N

PAX5 mouse monoclonal antibody, clone 8F9, Purified

Product Images

PAX5 monoclonal antibody clone 8F9. Western Blot analysis of PAX5 expression in IMR-32.

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  Detection limit for recombinant GST tagged PAX5 is approximately 0.03ng/ml as a capture antibody.

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  Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 8F9
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: PAX5 (NP_057953, 192 a.a. ~ 302 a.a) partial recombinant protein with GST tag.
• Specificity: This antibody recognizes PAX5
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens paired box 5 (PAX5), transcript variant 1
• Database Link:
  Entrez Gene 5079 Human
• Background: Pax-5 is a B cell specificactivator protein (BSAP). In the early stages of B cell developmemt, Pax-5 influences the expression of several B-cell-specific genes, such as CD19 and CD20. Pax-5 is expressed primarily inpro-, pre-, and mature B cells, but not inplasma cells. Interestingly, Pax-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis. Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis. This indicates that Pax-5 is important in B cell development, but may also have a role in proper neuronal development.
• Synonyms: Pax-5, BSAP

Reference Data

• Protein Families: Transcription Factors