NEK2 (331-445) Mouse Monoclonal Antibody [Clone ID: 2F6]

CAT#: AM31131PU-N

NEK2 (331-445) mouse monoclonal antibody, clone 2F6, Purified

Product Images

Western Blot analysis of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody clone 2F6.

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  Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody.

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  Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 2F6
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Immunoprecipitation.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: NEK2 antibody was raised against NEK2 (AAH43502, 331 a.a. ~ 445 a.a) partial recombinant protein with GST tag.
• Specificity: This antibody recognizes Human NEK2 at aa 331-445.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 3
• Database Link:
  Entrez Gene 4751 Human
• Background: NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.
• Synonyms: NLK1, NimA-related protein kinase 2, HSPK 21

Reference Data

• Protein Families: Druggable Genome, Protein Kinase