IL2 Receptor alpha (IL2RA) Mouse Monoclonal Antibody [Clone ID: B-B10]

CAT#: AM31169BT-N

IL2 Receptor alpha (IL2RA) mouse monoclonal antibody, clone B-B10, Biotin

Product Images

A typical staining pattern with the B-B10 monoclonal antibody of PHA activated lymphocytes.

Specifications

Product Data

• Clone Name: B-B10
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl to label 10⁶ cells or 100 µl of whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: PHA activated T cells.
  Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells
• Specificity: Recognises the Interleukin 2 Receptor a chain (IL-2 Ra), a 55 kDa protein.
  This antibody recognizes only membrane bound Human IL-2 Ra.
• Formulation: PBS
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore with 1.0 ml deionised water.
• Purification: Ion Exchange Chromatography
• Conjugation: Biotin
• Storage: Store the antibody undiluted after reconstitution at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens interleukin 2 receptor, alpha (IL2RA), transcript variant 1
• Database Link:
  Entrez Gene 3559 Human
• Background: IL-2R (T-cell growth factor receptor) consists of a chain of 55kDa (also known as Tac antigen or p55 or alpha chain) and a p75 or beta chain. IL-2RA is over-expressed on the surface of activated Tcells, HTLV-1 or -2 positive T-cell lines, and moderately expressed on activated B-cells, NK-cells, and macrophages. CD25 molecule alone appears to function as a low affinity receptor and associates wtih CD122 (IL-2R beta chain, p75) and CD132 (common gamma chain) to form the high affinity IL-2 receptor complex.
• Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen
• Note: Kd: 0.4 nM

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Jak-STAT signaling pathway