CD130 (IL6ST) Mouse Monoclonal Antibody [Clone ID: B-S12]

CAT#: AM31178AF-N

CD130 (IL6ST) mouse monoclonal antibody, clone B-S12, Azide Free

Product Images

A typical staining pattern with the B-S12 monoclonal antibody of Eahy 926 cell line.

Specifications

Product Data

• Clone Name: B-S12
• Applications: FC, FN, IP, WB
• Recommended Dilution: Western blot.
  Immunoprecipitation.
  Flow Cytometry.
  Functional Assay: Activates cells carrying gp130.
  Induces gp130 activation, Jak 1, Jak 2, Stat 1 and Stat 3 phosphorylation.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Natural soluble gp130.
  Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells.
• Specificity: Recognizes the soluble antigen.
  Recognizes the Gp130, common subunit for IL-6, IL-11, OSM, LIF, CNTF, CT-1 receptors, a 130-140 kDa protein.
• Formulation: Phosphate-buffered saline without Carrier and preservatives.
  This prodcut is sterile-filtered through 0.22 µm and treated to remove Endotoxins.
  State: Azide Free
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Ion Exchange Chromatography
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens interleukin 6 signal transducer (IL6ST), transcript variant 1
• Database Link:
  Entrez Gene 3572 Human
• Background: IL-6 activates intracellular signaling through binding a receptor consisting of an 80 kDa ligand-binding protein (IL-6R) and a second protein of 130 kDa. IL-6 first binds to IL-6R which subsequently associates with a gp130 dimer. The active signaling complex consists of at minimum IL-6, IL-6R and a dimer of two gp130 proteins that are linked by a disulfide bond. A soluble form of IL-6R is generated by proteolytic cleavage of the membrane-bound precursor and can function as an agonistic molecule that can actively participate in cell-tocell signaling. The second subunit of the IL-6 complex, gp130, also functions as a component of several additional receptor complexes including leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and IL-11. LIF binds to the LIF receptor with low affinity and to a complex of the LIF receptor and gp130 with high affinity, while OSM appears to bind to gp130 with low affinity and to a complex of gp130 and the LIF receptor with high affinity.
• Synonyms: Interleukin-6 receptor subunit beta, IL-6R-beta, IL6 receptor beta

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway