CD40 Mouse Monoclonal Antibody [Clone ID: B-B20]

CAT#: AM31223AF-N

CD40 mouse monoclonal antibody, clone B-B20, Azide Free

Product Images

Labelling on Frozen Section (Acetone Fixed) of Human colic mucosa with B-B20 diluted at 1/100 (background coloration by hematoxylin).

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  A typical staining pattern with the B-B20 monoclonal antibody of lymphocytes

Specifications

Product Data

• Clone Name: B-B20
• Applications: FC, FN, IHC
• Recommended Dilution: Flow cytometry.
  Functional Assay: Stimulates B-cell proliferation together with IL-4.
  Immunochemistry on Frozen Sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Raji cell line.
• Specificity: This antibody recognises the Bp50 antigen, a 48 kDa protein.
• Formulation: Phosphate-buffered saline.
  Sterile-filtered through 0.22 µm.
  Carrier and preservative free.
  State: Azide Free
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Ion Exchange Chromatography
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD40 molecule (CD40), transcript variant 1
• Database Link:
  Entrez Gene 958 Human
• Background: CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.
• Synonyms: TNFRSF5, CDw40, Bp50

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Allograft rejection, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Cytokine-cytokine receptor interaction, Primary immunodeficiency, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Viral myocarditis