CD14 Mouse Monoclonal Antibody [Clone ID: B-A8]

CAT#: AM31358PU-N

CD14 mouse monoclonal antibody, clone B-A8, Purified

Product Images

A typical staining pattern with the B-A8 monoclonal antibody of monocytes.

Specifications

Product Data

• Clone Name: B-A8
• Applications: ELISA, FC, IHC
• Recommended Dilution:

  Flow Cytometry: Use 10 µl to label 10e6 cells or 100 µl of whole blood.
  ELISA: This clone has been used as a Capture antibody in a Sandwich ELISA for analysis of Human CD14 in combination with clone MEM-18.
  Immunohistochemistry on Paraffin Sections: 1/200
  Positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side using this clone antibody.

• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Monocytes.
• Specificity: Recognises the lipopolysaccharide receptor (LPS R), a 55 kDa protein.
• Formulation: PBS containing 1% BSA as stabilizer and 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction
• Purification: Ion Exchange Chromatography
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD14 molecule (CD14), transcript variant 1
• Database Link:
  Entrez Gene 929 Human
• Background: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
• Synonyms: CD14

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, MAPK signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Toll-like receptor signaling pathway