FLIP (CFLAR) Mouse Monoclonal Antibody [Clone ID: 2F12]

CAT#: AM31373PU-N

FLIP (CFLAR) mouse monoclonal antibody, clone 2F12, Purified

Product Images

CFLAR monoclonal antibody, clone 2F12 Western Blot analysis of CFLAR expression in IMR-32.

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  Detection limit for recombinant GST tagged CFLAR is approximately 0.3ng/ml as a capture antibody.

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  Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to CFLAR on HeLa cell. [antibody concentration 10 ug/ml]

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Specifications

Product Data

• Clone Name: 2F12
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: CFLAR antibody was raised against recombinant protein
• Specificity: This antibody reacts to CFLAR / FLIP.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 0.47 mg/ml
• Purification: Affinity chromatography on Protein A
• Gene Name: Homo sapiens CASP8 and FADD like apoptosis regulator (CFLAR), transcript variant 2
• Database Link:
  Entrez Gene 8837 Human
• Background: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPa, b, g, and d, respectively.
• Synonyms: c-FLIP, CLARP, MRIT, CASH, FLAME-1, CASP8AP1, Usurpin

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Apoptosis