Ebi3 Rat Monoclonal Antibody [Clone ID: DNT27]

CAT#: AM31375PU-N

Ebi3 rat monoclonal antibody, clone DNT27, Aff - Purified

Product Images

Recombinant Mouse EBI3/p28 (covalently linked IL-27) protein (Lane 1) and Mouse bone marrow-derived dendritic cells stimulated with LPS (Lane 2) or left untreated (Lane 3) were analyzed by SDS-PAGE followed by Immunoblotting with 5 ug/ml of Rat anti-Mouse EBI3 antibody (DNT27). Bands were visualized with horseradish peroxidase (HRP)-conjugated Goat anti-Rat IgG.

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  Human Lyphoid-Dendritic Cells: Formalin-Fixed, Paraffin-Embedded (FFPE).

Specifications

Product Data

• Clone Name: DNT27
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot.
• Reactivities: Human, Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: EBI3 / IL-27B antibody was raised against mouse EBI3
• Specificity: The monoclonal antibody DNT27 reacts with Mouse Epstein-Barr Virus-induced gene 3 (EBI3).
• Formulation: Aqueous buffer containing 0.09% Sodium Azide and stabilizer if necessary
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: 0.5 mg/ml
• Purification: Affinity Chromatography
• Gene Name: Mus musculus Epstein-Barr virus induced gene 3 (Ebi3)
• Database Link:
  Entrez Gene 10148 HumanEntrez Gene 50498 Mouse
• Background: EBI3 is a 229 amino acid cytokine belonging to the type I cytokine receptor family (type 3 subfamily) with a signal peptide, 2 potential N-linked glycosylation sites, an LSDWS motif, 2 fibronectin type-III domains. It is usually induced due to infection of B lymphocytes by Epstein-Barr virus and due to expression of an EBV encoded latent membrane protein. EBI3 is a subunit of two heterodimeric cytokines interleukin-27 (IL27) and IL35. It is a secreted glycoprotein associated with calnexin and is a heterodimer with an alpha and a beta subunit. It is also known to interact with SQSTM1 and is responsible for the rapid clonal expansion of naive but not memory CD4 (+) T-cells. It strongly synergizes with IL-12 and trigger IFN-gamma production of naive CD4 (+) T-cells. It mediates its biologic effects through the cytokine receptor WSX-1/TCCR. It triggers signaling in T cells, B cells, and myeloid cells and along with p35 it acts as an important immunomodulator. It is usually expressed in various hemopoietic tissues and cell lines.
• Synonyms: IL-27 subunit beta, IL-27B