CEACAM5 Mouse Monoclonal Antibody [Clone ID: B465M]

CAT#: AM31404PU-N

CEACAM5 mouse monoclonal antibody, clone B465M, Purified

Specifications

Product Data

• Clone Name: B465M
• Applications: ELISA, IHC
• Recommended Dilution: ELISA.
  Immunohistochemistry.
  Staining in Immunohistochemistry:
  Brightness 80
  Cytoplasmic staining 3+
  Membrane staining 2+
  Background 1+
  Staining of mucus 2+
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human natural tumor-derived purified CEA antigen
• Specificity: CEA, epitope specificity group V.
  Does not crossreact with Human leukocytes in Cytofluorometry.
• Formulation: 10mM Tris, pH 7.5 containing 0.15M Sodium Chloride and
  0.05% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction (> 90% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Ion Exchange Chromatography
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens carcinoembryonic antigen related cell adhesion molecule 5 (CEACAM5), transcript variant 1
• Database Link:
  Entrez Gene 1048 Human
• Background: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). CEACAM6 expression is strongly up-regulated already during early stages of adenocarcinoma formation (3). The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
• Synonyms: CEA, Carcinoembryonic antigen