Chromogranin A (CHGA) (19-131) Mouse Monoclonal Antibody [Clone ID: 3A5]

CAT#: AM31705PU-N

Chromogranin A (CHGA) (19-131) mouse monoclonal antibody, clone 3A5, Purified

Product Images

Cell lysates of A549, 293T and Mouse kidney (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Chromogranin A (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  CHGA antibody staining of Formalin-Fixed, Paraffin-Embedded Human Adrenal at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  CHGA antibody staining of Formalin-Fixed, Paraffin-Embedded Human Colon at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: 3A5
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human Chromogranin A (19-131aa) purified from E. coli.
• Specificity: Recognizes CHGA / Chromogranin A.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens chromogranin A (CHGA), transcript variant 1
• Database Link:
  Entrez Gene 12652 MouseEntrez Gene 1113 Human
• Background: The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.
• Synonyms: Chromogranin-A, CgA, CHGA, SP-I

Reference Data

• Protein Families: Druggable Genome, Secreted Protein