RHOA Mouse Monoclonal Antibody [Clone ID: 1B12]

CAT#: AM31718PU-N

RHOA mouse monoclonal antibody, clone 1B12, Purified

Product Images

Western Blot analysis of RHOA expression in HL-60 cell lysate.

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  Western Blot analysis of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate (21.8 KDa). Lane 2: Non-transfected lysate.

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  Detection limit for recombinant GST tagged RHOA is approximately 10ng/ml as a capture antibody.

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  Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Small Intestine, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to RHOA on HeLa cell at 10 ug/ml.

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Specifications

Product Data

• Clone Name: 1B12
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: RHOA antibody was raised against RHOA (AAH01360, 1 a.a. ~ 194 a.a) full length recombinant protein with GST tag.
• Specificity: Recognizes RhoA.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store the antibody (in aliquots) at -20° or -80°C.
  Avoid freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens ras homolog family member A (RHOA), transcript variant 1
• Database Link:
  Entrez Gene 387 Human
• Background: RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila).
• Synonyms: ARH12, ARHA, RHO12

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Neurotrophin signaling pathway, Pathogenic Escherichia coli infection, Pathways in cancer, Regulation of actin cytoskeleton, T cell receptor signaling pathway, TGF-beta signaling pathway, Tight junction, Vascular smooth muscle contraction, Wnt signaling pathway