HIF1 beta (ARNT) Mouse Monoclonal Antibody [Clone ID: 3D10]

CAT#: AM31721PU-N

HIF1 beta (ARNT) mouse monoclonal antibody, clone 3D10, Purified

Product Images

Western Blot analysis of ARNT expression in Hela nuclear extract.

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  Western Blot analysis of ARNT expression in transfected 293T cell line.

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  Detection limit for recombinant GST tagged ARNT is approximately 0.03 ng/ml as a capture antibody.

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  Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal ARNT antibody on HeLa cell at 10 ug/ml.

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Specifications

Product Data

• Clone Name: 3D10
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: ARNT (AAH60838, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag.
• Specificity: Recognizes HIF1 beta / ARNT.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store the antibody (in aliquots) at -20° or -80°C.
  Avoid freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 4
• Database Link:
  Entrez Gene 405 Human
• Background: Transcriptional responses to many environmental and developmental stimuli are mediated by the basic helix-loop-helix (bHLH)-PAS family of proteins, which form homodimers that bind regulatory DNA sequences. ARNT/HIF-1 beta regulates the expression of different sets of genes in response to distinct environmental challenges. ARNT forms a complex with arylhydrocarbon receptor (AHR) to activate expression of genes encoding P450. Once AHR binds it ligand, it associates with ARNT for transport into the nucleus. ARNT also dimerizes with HIF-1alpha, -2 alpha or -3 alpha in response to low oxygen concentration to induce the expression of hypoxia-regulated genes encoding vascular endothelial growth and other genes. The expression of ARNT/HIF-1beta remains fairly constant and the protein is found in the nucleus and the cytoplasm.
• Synonyms: BHLHE2, Dioxin receptor, Hypoxia-inducible factor 1 beta, HIF-1 beta

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma