CD247 (1-165) Mouse Monoclonal Antibody [Clone ID: 4A12-F6]

CAT#: AM31735PU-N

CD247 (1-165) mouse monoclonal antibody, clone 4A12-F6, Purified

Product Images

Western Blot analysis of CD247 expression in Jurkat cell lysate.

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  Western Blot analysis of CD247 expression in transfected 293T cell line. Lane 1: CD247 transfected lysate (Predicted MW: 18.7 KDa). Lane 2: Non-transfected lysate.

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  Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunoprecipitation of CD247 transfected lysate using anti-CD247 monoclonal antibody and Protein A Magnetic Bead.

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Specifications

Product Data

• Clone Name: 4A12-F6
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Immunoprecipitation.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CD247 antibody was raised against CD3Z (AAH25703, 1 a.a. ~ 165 a.a) full length recombinant protein with GST tag.
• Specificity: Recognizes CD247 / CD3Z at aa 1-165.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store the antibody (in aliquots) at -20°C or -80°C.
  Avoid freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD247 molecule (CD247), transcript variant 2
• Database Link:
  Entrez Gene 919 Human
• Background: CD3 consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta). The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). It is involved in signal transduction to the T cell interior following antigen recognition, which culminates in cytokine release, cellular proliferation. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
• Synonyms: T3Z, TCRZ, CD3 zeta chain

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway