CREB1 Mouse Monoclonal Antibody [Clone ID: 3F1-1B2]

CAT#: AM31739PU-N

CREB1 mouse monoclonal antibody, clone 3F1-1B2, Purified

Product Images

Western Blot analysis of CREB1 expression in transfected 293T cell line. Lane 1: CREB1 transfected lysate (37 KDa). Lane 2: Non-transfected lysate.

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  Detection limit for recombinant GST tagged CREB1 is approximately 1ng/ml as a capture antibody.

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  Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to CREB1 on HeLa cell at 10 ug/ml.

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Specifications

Product Data

• Clone Name: 3F1-1B2
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CREB1 antibody was raised against cREB1 (AAH10636, 1 a.a. ~ 342 a.a) full length recombinant protein with GST tag.
• Specificity: Recognizes CREB1
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Purification: Protein A Chromatography
• Storage: Store the antibody (in aliquots) at -20°C or -80°C.
  Avoid freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens cAMP responsive element binding protein 1 (CREB1), transcript variant A
• Database Link:
  Entrez Gene 1385 Human
• Background: CREB (cAMP response element binding protein1), a DNA binding protein, is a member of the leucine zipper family of transcription factors. CREB interaction with DNA requires phosphorylation at Ser 119 by activated cAMP protein kinase A. Once phosphorylated CREB binds to an 8 base enhancer know as the cAMP response element (CRE) containing a conserved core sequence TGACG. CREB also interacts with CBP (CREB binding protein) and RNA polymerase II when phosphorylated on Ser 133. Different isoforms of this protein are the result of two transcript variants.
• Synonyms: CREB-1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Antigen processing and presentation, Huntington's disease, Melanogenesis, Prostate cancer