ErbB 3 (ERBB3) Mouse Monoclonal Antibody [Clone ID: 3F12]

CAT#: AM31753PU-N

ErbB 3 (ERBB3) mouse monoclonal antibody, clone 3F12, Purified

Product Images

Detection limit for recombinant GST tagged ERBB3 is approximately 3ng/ml as a capture antibody.

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  Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Placenta, Trophoblast: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 3F12
• Applications: ELISA, IHC
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: ERBB3 (AAH02706, 21 a.a. ~ 332 a.a) full length recombinant protein with GST tag.
• Specificity: Recognizes Human ERBB3 / HER3
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 (ERBB3), transcript variant s
• Database Link:
  Entrez Gene 2065 Human
• Background: ERBB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of the ERBB3 gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form.
• Synonyms: ERBB-3, c-erbB-3, HER-3

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Protein Kinase, Secreted Protein, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Calcium signaling pathway, Endocytosis, ErbB signaling pathway