SNF5 (SMARCB1) (81-181) Mouse Monoclonal Antibody [Clone ID: 3E10]

CAT#: AM31756PU-N

SNF5 (SMARCB1) (81-181) mouse monoclonal antibody, clone 3E10, Purified

Product Images

Western Blot analysis of SMARCB1 expression in PC-12 cell lysate.

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  Western Blot analysis of SMARCB1 expression in Hela nuclear extract.

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  Detection limit for recombinant GST tagged SMARCB1 is approximately 0.03ng/ml as a capture antibody.

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  Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 3E10
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: SMARCB1 (NP_003064, 81 a.a. ~ 181 a.a) partial recombinant protein with GST tag
• Specificity: Recognizes Human SMARCB1 at aa 81-181.
  Other species not tested.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 (SMARCB1), transcript variant 2
• Database Link:
  Entrez Gene 20587 MouseEntrez Gene 361825 RatEntrez Gene 6598 Human
• Background: Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
• Synonyms: Integrase interactor 1 protein, INI1, SNF5 homolog, SNF5L1, BAF47

Reference Data

• Protein Families: Transcription Factors