c Kit (KIT) (41-140) Mouse Monoclonal Antibody [Clone ID: 5F6]

CAT#: AM31759PU-N

c Kit (KIT) (41-140) mouse monoclonal antibody, clone 5F6, Purified

Product Images

Western Blot analysis of KIT expression in transfected 293T cell line by KIT monoclonal antibody, clone 5F6.

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  Detection limit for recombinant GST tagged KIT is 0.03 ng/ml as a capture antibody.

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  Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 5F6
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: KIT antibody was raised against kIT (NP_000213, 41 a.a. ~ 140 a.a) partial recombinant protein with GST tag.
• Specificity: This antibody reacts to KIT at aa 41-140.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant 1
• Database Link:
  Entrez Gene 3815 Human
• Background: KIT encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Somatic gain-of-function mutations in the extracellular domain, kinase domains 1 and 2 are found in gastrointestinal stromal tumors (GISTs). Mutations in c-KIT gene are also associated with mast cell disease, acute myelogenous lukemia, and piebaldism. Tyrosine kinase inhibitor STI571 has been shown to inhibit GIST cells that express mutated c-KIT.
• Synonyms: SCFR, KIT

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Protein Kinase, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer