TNF alpha (TNF) (1-234) Mouse Monoclonal Antibody [Clone ID: M1-C4]

CAT#: AM31768PU-N

TNF alpha (TNF) (1-234) mouse monoclonal antibody, clone M1-C4, Purified

Product Images

TNF monoclonal antibody,. Western Blot analysis of TNF expression in rat liver.

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  Detection limit for recombinant GST tagged TNF is approximately 0.3ng/ml as a capture antibody.

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  Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: M1-C4
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: TNF (AAH28148, 1 a.a. ~ 234 a.a) full length recombinant protein with GST tag
• Specificity: This antibody reacts to Human TNF at aa 1-234.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens tumor necrosis factor (TNF)
• Database Link:
  Entrez Gene 24835 RatEntrez Gene 7124 Human
• Background: Tumor Necrosis Factor (TNF) is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. TNF is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus