EP300 Mouse Monoclonal Antibody [Clone ID: 1B1]

CAT#: AM31780PU-N

EP300 mouse monoclonal antibody, clone 1B1, Purified

Product Images

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

• 

  Immunofluorescence of monoclonal antibody to EP300 on HeLa cell. [antibody concentration 10 ug/ml]

Specifications

Product Data

• Clone Name: 1B1
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: EP300 antibody was raised against eP300 (NP_001420, 731 a.a. ~ 831 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to EP300 / p300.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody at -20°C to -80°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens E1A binding protein p300 (EP300)
• Database Link:
  Entrez Gene 2033 Human
• Background: EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
• Synonyms: p300 HAT, KAT3B, Histone acetyltransferase p300

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Huntington's disease, Jak-STAT signaling pathway, Long-term potentiation, Melanogenesis, Notch signaling pathway, Pathways in cancer, Prostate cancer, Renal cell carcinoma, TGF-beta signaling pathway, Wnt signaling pathway