HDAC1 Mouse Monoclonal Antibody [Clone ID: 1D6]

CAT#: AM31795PU-N

HDAC1 mouse monoclonal antibody, clone 1D6, Purified

Product Images

Western Blot analysis of HDAC1 expression in HeLa nuclear extract.

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  Western Blot analysis of HDAC1 expression in NIH/3T3 cell lysate.

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  Western Blot analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody.

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  Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.

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  Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml]

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Specifications

Product Data

• Clone Name: 1D6
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: HDAC1 antibody was raised against hDAC1 (AAH00301, 1 a.a. ~ 483 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to HDAC1.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody at -20°C to -80°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens histone deacetylase 1 (HDAC1)
• Database Link:
  Entrez Gene 297893 RatEntrez Gene 433759 MouseEntrez Gene 3065 Human
• Background: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
• Synonyms: Histone deacetylase 1, HD1, RPD3L1

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Huntington's disease, Notch signaling pathway, Pathways in cancer