NM23A (NME1) Mouse Monoclonal Antibody [Clone ID: 1D7]

CAT#: AM31804PU-N

NM23A (NME1) mouse monoclonal antibody, clone 1D7, Purified

Product Images

NME1 monoclonal antibody LS-B6104. Western Blot analysis of NME1 expression in HeLa.

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  Western Blot analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody

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  Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.

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  Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml]

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Specifications

Product Data

• Clone Name: 1D7
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Immunoprecipitation.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: NME1 antibody was raised against nME1 (NP_000260, 43 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to NME1.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens NME/NM23 nucleoside diphosphate kinase 1 (NME1), transcript variant 2
• Database Link:
  Entrez Gene 4830 Human
• Background: NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. NME1 encodes the 'A' isoform of nucleoside diphosphate kinase (NDK). NDK exists as a hexamer composed of the 'A' (NME1) and 'B' (encoded by NME2) isoforms. Mutations in NME1 have been identified in aggressive neuroblastomas.
• Synonyms: NME1, NDPKA, NM23, nm23-H1, GAAD

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism