Cytokeratin 17 (KRT17) (full length) Mouse Monoclonal Antibody [Clone ID: 2D4-1G9]

CAT#: AM31808PU-N

Cytokeratin 17 (KRT17) (full length) mouse monoclonal antibody, clone 2D4-1G9, Purified

Product Images

Western Blot analysis of KRT17 expression in A-431 cell lysate.

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  Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to KRT17 on HeLa cell. [antibody concentration 25 ug/ml]

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  Immunoprecipitation of KRT17 transfected lysate using anti-KRT17 monoclonal antibody and Protein A Magnetic Bead.

Specifications

Product Data

• Clone Name: 2D4-1G9
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 25 µg/ml.
  Immunohistochemistry on Paraffin Sections: 1.25 µg/ml.
  Immunoprecipitation.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: KRT17 antibody was raised against kRT17 (AAH00159, 1 a.a. ~ 433 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to KRT17 / Cytokeratin 17 at aa 1-433.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Upon receipt, store (in aliquots) at -20°C to -80°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens keratin 17 (KRT17)
• Database Link:
  Entrez Gene 3872 Human
• Background: May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
  Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).
  Defects in KRT17 are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
  Defects in KRT17 are a cause of steatocystoma multiplex (SM) [MIM:184500]. SM is a disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs.
  Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
• Synonyms: Cytokeratin-17, Keratin-17, Keratin 17, KRT17, CK17, K17