DNA Ligase I (LIG1) Mouse Monoclonal Antibody [Clone ID: 10G12]

CAT#: AM31811PU-N

DNA Ligase I (LIG1) mouse monoclonal antibody, clone 10G12, Purified

Product Images

Western Blot analysis of LIG1 expression in HeLa nuclear extract.

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  Detection limit for recombinant GST tagged LIG1 is approximately 0.3ng/ml as a capture antibody.

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  Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 10G12
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: LIG1 antibody was raised against lIG1 (NP_000225, 810 a.a. ~ 920 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to LIG1.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody at -20°C to -80°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens DNA ligase 1 (LIG1), transcript variant 1
• Database Link:
  Entrez Gene 3978 Human
• Background: LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.
• Synonyms: LIG1, LIG-1, DNA ligase 1, EC=6.5.1.1, DNA ligase I

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair, DNA replication, Mismatch repair, Nucleotide excision repair