PAK1 Mouse Monoclonal Antibody [Clone ID: 1E11]

CAT#: AM31813PU-N

PAK1 mouse monoclonal antibody, clone 1E11, Purified

Product Images

Detection limit for recombinant GST tagged PAK1 is approximately 0.03ng/ml as a capture antibody.

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  Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 1E11
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: PAK1 (NP_002567, 191 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody recognizes Human PAK1. Other species not tested.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 1
• Database Link:
  Entrez Gene 5058 Human
• Background: PAK1, a PAK (STE20) type protein kinase, is an effector for the small GTP binding proteins Cdc42 and Rac in the regulation of cell motility and morphology. The activated protein has been shown to act on a variety of targets, including actin-binding protein filamin A, myosin light chain kinase, histone H3 and the stress-activated protein kinase JNK1. The PAK1 gene has been mapped to chromosome 11q13, a region of amplification in a subset of estrogen receptor positive breast carcinomas prone to metastasis.
• Synonyms: PAK 1, PAK-1, Alpha-PAK, PAK alpha, p21-activated kinase 1, p65-PAK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway