S100A9 Mouse Monoclonal Antibody [Clone ID: CF-557]

CAT#: AM31839PU-N

S100A9 mouse monoclonal antibody, clone CF-557, Purified

Product Images

Cell Source: Human Peripheral Blood Leukocytes labeled with and PE Secondary Antibody. Percentage of cells stained above control: 74.7%

Specifications

Product Data

• Clone Name: CF-557
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 2.0 µg/10⁶ cells.
  Positive Control: Human peripheral blood leukocytes.
  Immunofluorescence (Reported).
  Western blot (Reported).
  Immunohistochemistry (Reported).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified granulocyte antigen of Human origin.
• Specificity: This antibody Binds to Human Calgranulin B (S100A9). Other species not tested.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Ascitic fluid
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Protein G Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens S100 calcium binding protein A9 (S100A9)
• Database Link:
  Entrez Gene 6280 Human
• Background: Calgranulin B, also known as MRP-14 and S100A9 is a member of the S100 family of proteins containing 2 EF hand (alpha helix, turn, alpha helix structure) calcium binding motifs. S100 proteins are localized in the cytoplasm and /or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation.
  MRP-14 (S100A9) forms a heterodimeric complex with MRP-8 (S100A8) in the cytosol of monocyte and neutrophil cell types circulating in peripheral blood. Calgranulin B is found in elevated levels in the serum of cystic fibrosis cases and is also expressed in the skin of patients with psoriasis, eczematous dermatitis and squamous cell carcinoma. Calgranulin B is also expressed on macrophages in acute inflamed tissues (peridontitis, contact excema).
• Synonyms: S100-A9, CAGB, MRP-14
• Note: Protocol: Tests Results
  Tissue Distribution by Flow Cytometry Analysis:
  Cell Concentration: 1x10⁶ cells per tests.
  Antibody Concentration Used: 2.0 μg/10⁶ cells.
  Isotypic Control (shaded): Purified Mouse IgG1.