p75 NGF Receptor (NGFR) Mouse Monoclonal Antibody [Clone ID: ME20.4]

CAT#: AM31842PU-N

p75 NGF Receptor (NGFR) mouse monoclonal antibody, clone ME20.4, Purified

Specifications

Product Data

• Clone Name: ME20.4
• Applications: FC, IF, IHC, IP, NEUT, WB
• Recommended Dilution: Flow Cytometry.
  Immunohistochemistry on frozen and paraffin sections.
  Immunoprecipitation.
  Immunofluorescence.
  Functional Assay.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: WM245 melanoma cells
• Specificity: Anti-Neuronal Growth Factor Receptor (NGFR), p75, low affinity receptor. Detects a protein with a molecular weight of 75 kDa.
• Formulation: PBS (20mM), 0.14M NaCl, pH 7.3. containing 0.09%
  sodium azide (NaN3) as preservative and 0.2% gelatin as stabilizer
  State: Purified
  State: Liquid purified Ig fraction
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens nerve growth factor receptor (NGFR)
• Database Link:
  Entrez Gene 4804 Human
• Synonyms: TNFRSF16
• Note: Protocol: Flow Cytometry Protocol:
  1. Add 10 μl of antibody to 1 x 10e6 cells.
  2. Incubate 30 minutes on ice in PBS containing 2-5% BSA.
  3. Wash via centrifugation and add second-step antibody at appropriate dilution.
  4. Incubate 20-30 minutes and wash again.
  5. Analyze by flow cytometry.
  Positive Control Cell Line: HS294T from ATCC
  Procedure For General Staining Using Flow Cytometry: (For Non-Adherent Cells):
  1. Add 0.3-1.0 μg anti-NGFR FITC or PE in 10 μl to one million cells in 100 μl PBS, 2% BSA.
  2. Incubate on ice for 30 minutes.
  3. Add 1.0 ml PBS, BSA and centrifuge for 5 minutes at 500xg to wash cells.
  4. Suction off PBS, BSA and add 1.0 ml fresh PBS, BSA.
  5. Analyze by flow cytometry.
  6. HS294 T-cell line from ATCC can be used for positive control.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Neurotrophin signaling pathway