PF4 Mouse Monoclonal Antibody [Clone ID: KKO]

CAT#: AM31851PU-N

PF4 mouse monoclonal antibody, clone KKO, Purified


USD 475.00

2 Weeks*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name KKO
Applications ELISA, WB
Recommended Dilution ELISA: Use at 0.08 μg/ml following coating plate with PF4/ heparin complex. This can be done by incubating PF4 with an excess of heparin (100-200 U/ml), while coating the plate.
Western blot.
Reactivities Human
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Human Factor IV purified from the supernatant of Thrombin activated platelets.
Specificity This antibody is specific for Human Platelet Factor IV/heparin complexes.
AM31851PU-N (clone KKO), unlike AM31850PU-N (clone RTO) which binds PF4 alone, is specific for PF4/heparin complexes and is seen to have similar binding properties as auto-antibodies found in HIT/HITT.
Formulation PBS
State: Purified
State: Liquid purified IgG fraction
Preservative: 0.02% Sodium Azide
Concentration lot specific
Purification Protein G Chromatography of Ascitic fluid
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens platelet factor 4 (PF4)
Background Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity.

Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies.
These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FCGIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation.
Synonyms PF-4, Iroplact, Oncostatin-A, CXCL4, SCYB4
Reference Data
Protein Families Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways Chemokine signaling pathway, Cytokine-cytokine receptor interaction

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.