Cd5 Rat Monoclonal Antibody [Clone ID: 53-7.3]

CAT#: AM31857PU-N

Cd5 rat monoclonal antibody, clone 53-7.3, Purified


USD 330.00

2 Weeks*

Size
    • 250 ug

Product Images

Specifications

Product Data
Clone Name 53-7.3
Applications FC, IHC, IP
Recommended Dilution This clone (53-7.3) has been reported to work in Immunohistochemistry on Acetone Fixed Frozen Sections and Zinc Fixed Paraffin Sections, Immunoprecipitation and Flow Cytometry.
Reactivities Human, Mouse
Host Rat
Isotype IgG2a
Clonality Monoclonal
Immunogen Mouse thymus or spleen from Balb/c mouse thymus or spleen.
Specificity This CD5 (Ly-1) Monoclonal Antibody is specific for the Mouse / Human CD5 antigen.

Test Results for tissue distribution by flow cytometry:
Cell concentration = 1x106 cells per test
Antibody concentration = 0.2 µg/106 cells

Percentage of cells stained above control:
95.9% - Thymus
39.19% -Spleen
3.66% - Bone Marrow
Formulation PBS containing 0.02% Sodium Azide as preservative
State: Purified
State: Liquid purified Ig fraction
Concentration 1.0 mg/ml
Purification Protein G Chromatography
Gene Name Mus musculus CD5 antigen (Cd5)
Background CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
Synonyms CD5, LEU1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.