MHC Class I H-2 Kk Mouse Monoclonal Antibody [Clone ID: H100-5/28]

CAT#: AM31876RP-N

MHC Class I H-2 Kk mouse monoclonal antibody, clone H100-5/28, PE

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Specifications

Product Data

• Clone Name: H100-5/28
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Test Results:
  Mouse Strain: CBA
  Cell Concentration : 1x10e6 cells per test.
  Antibody Concentration Used: 0.5 μg/10e6 cells.
  Isotypic Control: PE Mouse IgG2a,
  Cell Source Percentage of cells stained above control: Spleen: 97.3%
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This Monoclonal Antibody specifically reacts with the H-2Kk (Determinant H-2.m11) encoded MHC Class I antigen expressed on mouse strains of the H-2k haplotype.
  This antibody is also reported to react with H-2Kq and H-2r haplotypes. Class I antigens are expressed on all nucleated cells.
• Formulation: PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
  Label: PE
  State: Liquid purified Ig fraction
• Concentration: 0.1 mg/ml
• Purification: Protein G Chromatography
• Conjugation: PE
• Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta2- microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. Lymphocytes are highly positive whereas fibroblasts or neurons show only a low level of antigen.
• Synonyms: H2-K1, H2-K, H-2K(K)