Cd80 Rat Monoclonal Antibody [Clone ID: RMMP-1]

CAT#: AM31878RP-L

Cd80 rat monoclonal antibody, clone RMMP-1, Purified

Product Images

Cell Type: Spleen

Specifications

Product Data

• Clone Name: RMMP-1
• Applications: FC
• Recommended Dilution: Flow Cytometry (See Protocols).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody recognises the murine CD80 cell surface protein, also known as B7-1.
• Formulation: PBS containing 0.09% Sodium Azide as preservative and EIA grade BSA to bring total protein concentration to 4-5 mg/ml
  State: Purified
  State: Liquid purified IgG fraction
  Label: R-Phycoerythrin (R-PE)
• Concentration: 0.1 mg/ml
• Gene Name: Mus musculus CD80 antigen (Cd80)
• Database Link:
  Entrez Gene 12519 Mouse
• Background: CD80 is a member of the Ig superfamily, along with CD86 (B7-2), participates in T cell costimulation via interactions with CD28 and CTLA-4. CD80 is constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages; and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig. Expression of CD80 is greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours. It has been reported that activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours.
• Synonyms: CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1
• Note: Protocol: FLOW CYTOMETRY ANALYSIS:
  
  Method:
  1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
  2. Wash 2 times.
  3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
  4. To each tube, add ~1.0 μg* of AM31878RP-N or AM31878RP-L per 1x10e6 cells.
  5. Vortex the tubes to ensure thorough mixing of antibody and cells.
  6. Incubate the tubes at 4°C for 30 minutes.
  (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive).
  7. Wash 2 times at 4°C.
  8. Resuspend the cell pellet in 50 μl ice cold media B.
  9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
  
  Media:
  A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
  B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
  
  Results:
  Tissue Distribution by Flow Cytometry Analysis:
  Mouse Strain: C3H
  Cell Concentration : 1x10e6 cells per test
  Antibody Concentration Used: 1.0 μg/106 cells
  Isotypic Control: PE Rat IgG2a