ADAM9 / MDC9 Rat Monoclonal Antibody [Clone ID: 7D10]

CAT#: AM31879PU-N

ADAM9 / MDC9 rat monoclonal antibody, clone 7D10, Purified

Specifications

Product Data

• Clone Name: 7D10
• Applications: WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: Purified Recombinant Mouse ADAM9 extracellular domain.
• Specificity: This antibody detects both ADAM9 and ADAM15 in Western blotting.
• Formulation: 0.2 μm filtered PBS solution
  State: Purified
  State: Lyophilized purified IgG fraction of of Culture Supernatant
  Stabilizer: None
• Reconstitution Method: Restore with 200 µl sterile PBS and the final concentration is 500 µg/ml.
• Purification: Protein A/G Affinity Chromatography
• Background: ADAM9, also known as MDC9 or meltrin γ, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain. Like other membrane-anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Metturn expected for reprolysins, a disintegrin like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factorα (TNFα), the p75 TNF receptor, the β-amyloid protein precursor, and the c-kit ligand1, implying that it may participate in shedding of these membrane proteins. In fact, ADAM9 has been shown to shed membrane-anchored heparin-binding EGF-like growth factor. In addition, it also cleaves oxidized insulin B-chain and fibronectin. Besides its catalytic activity, ADAM9 functions as an adhesion molelcule through binding of its disintegrin domain to integrins such as αvβ5 and α6β1. The cytoplasmic domain of ADAM9 interacts with Src homology 3 (SH3)containing proteins and protein kinase C, and may mediate different signaling pathways. ADAM9 is widely expressed in tissues.
• Synonyms: KIAA0021, MCMP, MLTNG, Meltrin gamma