Pdcd1 Hamster Monoclonal Antibody [Clone ID: J43]

CAT#: AM31881PU-N

Pdcd1 hamster monoclonal antibody, clone J43, Purified

Product Images

Cell Source: Con A/IL2 activated T-Blasts from Balb/c spleen. Percentage of cells stained above control: 36.6%

Specifications

Product Data

• Clone Name: J43
• Applications: FC
• Recommended Dilution: Flow Cytometry.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: PD-1 cDNA transfectant.
  Donor: mouse ascites fluid.
  Fusion Partner: Mouse myeloma P3U1.
• Specificity: This Monoclonal Antibody reacts with Mouse PD-1 (Programmed Death-1).
• Formulation: PBS containing 0.02% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: 1.0 mg/ml
• Purification: Protein G Chromatography
• Gene Name: Mus musculus programmed cell death 1 (Pdcd1)
• Database Link:
  Entrez Gene 18566 Mouse
• Background: CD279 is 50-55kD membrane protein which is a member of the CD28 family, and functions mainly as a negative regulator of T-cell activation. CD279 has two specific ligands; CD274 (PD-L1) and CD273 (PD-L2), and their interaction is key in the balance between stimulatory and inhibitory signals needed for effective immune responses to microbes and self-tolerance. CD279 is inducibly expressed by T-cells, B-cells, NK-T-cells and monocytes upon activation. Loss of CD279 function has been associated with a number of autoimmune diseases, including rheumatoid arthritis, type I diabetes and ankylosing spondylitis. Recent studies suggest that CD279 could be targeted therapeutically in the treatment of HIV infection to reduce T-cell exhaustion.
• Synonyms: Protein PD-1, hPD-1, PDCD1
• Note: TEST RESULTS (Figure 1.)
  Tissue Distribution by Flow Cytometry Analysis:
  Mouse Strain: Balb/c
  Antibody Concentration Used: 2.0 µg/10e6 cells.
  Isotypic Control (shaded): Purified Hamster IgG.