CD45 / LCA Rat Monoclonal Antibody [Clone ID: IBL-5/25]
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Product Data | |
Clone Name | IBL-5/25 |
Applications | FC |
Recommended Dilution | Immunohistochemistry on aceton-fixed frozen sections. Immunoprecipitation. Flow Cytometry. TEST RESULTS: Mouse Strain: BALB/c Cell Concentration: 1x10e6 cells per tests Antibody Concentration Used: 0.1 μg/10e6 cells, Isotypic Control: PE-Rat IgG1 Cell Source Percentage of cells stained above control: Mesentric Lymph Node 99.8% |
Reactivities | Mouse |
Host | Rat |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | IL-3 dependent mast cells derived from WB- +/+ mice Donor: Wistar spleen Fusion Partner: X63.653. Ag8 |
Specificity | Anti-mouse CD45 monoclonal antibody detects CD45 (L-CA) which is a transmembrane phosphotyrosine phosphatase expressed on leukocytes. This mAb induces the in vitro clustering of mouse lymphocytes (both T and B cells). |
Formulation | PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Label: PE State: Liquid purified Ig fraction Label: R - Phycoerythrin |
Concentration | 0,1 mg/ml |
Purification | Affinity chromatography on Protein G |
Conjugation | PE |
Synonyms | PTPRC, Leukocyte common antigen, L-CA, T200 |
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