CD45 / LCA Rat Monoclonal Antibody [Clone ID: IBL-5/25]

CAT#: AM31886RP-N

CD45 / LCA rat monoclonal antibody, clone IBL-5/25, PE

Specifications

Product Data

• Clone Name: IBL-5/25
• Applications: FC
• Recommended Dilution: Immunohistochemistry on aceton-fixed frozen sections.
  Immunoprecipitation.
  Flow Cytometry.
  TEST RESULTS:
  Mouse Strain: BALB/c
  Cell Concentration: 1x10e6 cells per tests
  Antibody Concentration Used: 0.1 μg/10e6 cells,
  Isotypic Control: PE-Rat IgG1
  Cell Source Percentage of cells stained above control:
  Mesentric Lymph Node 99.8%
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: IL-3 dependent mast cells derived from WB- +/+ mice
  Donor: Wistar spleen
  Fusion Partner: X63.653. Ag8
• Specificity: Anti-mouse CD45 monoclonal antibody detects CD45 (L-CA) which is a transmembrane phosphotyrosine phosphatase expressed on leukocytes. This mAb induces the in vitro clustering of mouse lymphocytes (both T and B cells).
• Formulation: PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
  Label: PE
  State: Liquid purified Ig fraction
  Label: R - Phycoerythrin
• Concentration: 0,1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: PE
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200