Salivary alpha amylase (AMY1A) (172-246) Mouse Monoclonal Antibody [Clone ID: 2D4]

CAT#: AM31902PU-N

Salivary alpha amylase (AMY1A) (172-246) mouse monoclonal antibody, clone 2D4, Purified

Product Images

Western Blot analysis of AMY1A expression in Human pancreas using AMY1A monoclonal antibody, clone 2D4.

• 

  Human Salivary Gland, Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 2D4
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: AMY1A (NP_001008222, 172 a.a. ~ 246 a.a) partial recombinant protein with GST tag
• Specificity: Recognizes AMY1A at aa 172-246.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens amylase, alpha 1B (salivary) (AMY1B)
• Database Link:
  Entrez Gene 277 HumanEntrez Gene 276 Human
• Background: Amylases are secreted proteins that hydrolyze 1,4 alpha glucoside bonds in oligosaccharides and polysaccharides. They catalyze the first step in digestion of dietary starch and glycogen. Alternative splicing results in multiple transcript variants encoding the same protein.
  Salivary Amylase is a digestive enzyme secreted by salivary glands, it consists of a single polypeptide chain with a molecular weight of 56kDa (family B) or 62kDa (family A), the difference in molecular weight being due to the presence or absence of a carbohydrate moiety.
  The total serum concentration of amylase is about 120g/L of which about 50% is salivary amylase.
  Although salivary amylase and pancreatic amylase share 95% amino acid sequence homology, they differ in molecular size, isoelectricpoint and antigenic properties.
• Synonyms: Salivary alpha-amylase, AMY1A

Reference Data

• Protein Pathways: Metabolic pathways, Starch and sucrose metabolism