CEACAM1 Mouse Monoclonal Antibody [Clone ID: 2F6]

CAT#: AM31929PU-N

CEACAM1 mouse monoclonal antibody, clone 2F6, Purified

Product Images

Detection limit for recombinant GST tagged CEACAM1 is 3 ng/ml as a capture antibody.

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  Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 2F6
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein
• Specificity: This antibody recognizes Human CEACAM1.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens carcinoembryonic antigen related cell adhesion molecule 1 (CEACAM1), transcript variant 2
• Database Link:
  Entrez Gene 634 Human
• Background: Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1; also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily. It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region. The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation. There are three soluble and seven transmembrane isoforms. The three soluble forms also contain the first two C2-type Ig like domains (aa’s 145 317), with differences coming in the third C2-type Ig- like domain. The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57% aa identical to human CEACAM1; in the V-type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium.
• Synonyms: BGP, BGP1, Biliary glycoprotein 1

Reference Data

• Protein Families: Druggable Genome, Transmembrane