JNK1 (MAPK8) (318-427) Mouse Monoclonal Antibody [Clone ID: 2F3]

CAT#: AM31930PU-N

JNK1 (MAPK8) (318-427) mouse monoclonal antibody, clone 2F3, Purified

Product Images

Western Blot analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody, clone 2F3.

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  Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunoprecipitation of MAPK8 transfected lysate using anti-MAPK8 monoclonal antibody and Protein A magnetic bead, immunoblotted with MAPK8 rabbit polyclonal antibody.

Specifications

Product Data

• Clone Name: 2F3
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Immunoprecipitation.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant protein encoding amino acids 318 to 427 of NP_620637
• Specificity: This antibody recognizes Human MAPK8 / JNK1 at aa 318-427.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-a1
• Database Link:
  Entrez Gene 5599 Human
• Background: JNK1, a MAPK type protein kinase, regulates cell apoptosis, differentiation and growth in response to growth factors, inflammatory cytokines and cellular stress. JNK1 is phosphorylated by MKK4/MAP2K4 and MKK7/MAP2K7. Activated JNK1 translocates to the nucleus where it phosphorylates transcription factors, including c-Jun, ATF2, ELK1, Jun-D and NFAT4. At least four alternatively spliced transcript variants encoding distinct isoforms, 384 and 427 amino acids, have been identified.
• Synonyms: Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, Wnt signaling pathway