ABCC10 Rat Monoclonal Antibody [Clone ID: M7I-3]

CAT#: AM31987SU-N

ABCC10 rat monoclonal antibody, clone M7I-3, Supernatant

Specifications

Product Data

• Clone Name: M7I-3
• Applications: IF, IHC, WB
• Recommended Dilution: Western blotting: Use at 1/20-1/50 and anti-Rat-HRP.
  Immunocytochemistry: Use at 1/20-1/50 on Acetone Fixed Cytospin preparations.
  Immunohistochemisty on Frozen Sections: 1/20 on Acetone Fixed Frozen sections can be followed by incubation with Rabbit anti-Rat Ig (1/25) and a monoclonal Rat APAAP complex (1/50). Alternatively, after incubation with M7I-3 (use 1/20) and washing, slides can be incubated with biotinylated rabbit anti-Rat Ig (1/100) and streptavidin conjugated to horseradish peroxidase (1/500).
• Reactivities: Human
• Host: Rat
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A bacterial fusion protein of Human MRP7, containing amino acids 194-272 of the protein.
• Specificity: This Monoclonal antibody M7I-3 reacts with an internal epitope of MRP7 (ABCC10), an approximately 160 kD transmembrane protein that is related to the multidrug resistance protein MRP1.
  M7I-3 has potential value for the detection of MRP7-mediated drug-resistance in Human tumor samples.
• Formulation: State: Supernatant
  State: Serum Free Culture Supernatant
  Stabilizer: 0.7% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Storage: Store antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 89845 Human
• Background: The MRP family is represented by nine similar ABC transporters that have the ability to transport structurally diverse lipophilic anions and operate as chemical efflux pumps. MRP7 (multidrug resistance-associated protein 7, ATPbinding cassette sub-family C member 10) is a multi-pass membrane protein that belongs to the ABC transporter family (conjugate transporter subfamily). MRP7 is involved with the ATP-dependent transport of 17β-estradiol-D-17-β-glucuronide (E217βG). MRP7 is also probably involved in cellular detoxification through its lipophilic anion extrusion capabilities. MRP7 contains two ABC transmembrane type 1 domains and two ABC transporter domains. MRP7 likely has three isoforms. Isoform 2 is the most widely expressed, while isoform 1 is predominately expressed in the spleen.
• Synonyms: Multidrug resistance-associated protein 7, SIMRP7
• Note: 1 ml vials >> 200 tests